During the last decades the use of the conventional pituitary-derived FSH in superovulation (SOV) protocols have been associated with low yield and quality of the obtained embryos. The objective of the present study was to determine whether the stimulation with a single dose of bovine long-acting recombinant FSH (bscrFSH) could achieve an efficient SOV response compared to the conventional pituitary-derived FSH (FSH-p) in Brangus cattle. Sixteen healthy Brangus heifers (BW: 450± 50 kg; BCS: 3.5± 0.5) were randomly distributed into two groups: conventional [FSH-p: FSH from Purified Pig Pituitary Extract (Pluset, Buenos Aires, Argentina); n= 8] vs. recombinant group [bscrFSH: recombinant FSH (Cebitropin B, Concepción, Chile); n= 8]. The conventional SOV protocol was applied as follows: Day 0: intravaginal P4 device (CIDR: 1.38 gr) + 2.5 mg (i.m.) 17-β Estradiol + 50 mg P4 (i.m.); Day 4: 330 IU FSH-p/12 h intervals/4 d/8 decreasing doses, 60-60, 50-50, 35-35, 20-20, in total 8 injections; Day 6: 5th and 6th FSH-p dose + two PGF2α i.m. doses (500 µg D-cloprostenol each); Day 7: CIDR removal (p.m) at the 8th FSH-p dose application; Day 9: 0.0105 mg (i.m.) Buserelin Acetate (a.m) + AI (p.m.); Day 10: AI (a.m.). Regarding the recombinant group, the same protocol was applied with modifications (150 µg bscrFSH/24 h intervals/4 d/4 decreasing doses, 55-45-30-20, in total 4 injections). Ovarian structures [follicles (FL), corpora lutea (CL), and non-ovulated follicles (NOF)] were monitored by using ultrasonography on Day 8 (estrus; FL number) and Day 15 (embryo collection; CL and NOF number). Morphological embryo classification and quality were performed according to the IETS guidelines. The data were analysed by GLMM (SPSS® 25, USA). No statistical differences were observed between SOV protocols regarding FL, CL, or NOF (p> 0.05). Significant differences were observed in the number of total structures collected (15.8±2.9 vs. 6.0±1.3; bscrFSH-derived vs. FSH-p-derived SOV protocol, respectively; p= 0.01). Although no significant differences were observed in the number of non-transferable embryos (3.5±0.8 vs. 1.6±0.4; p=0.07), significant differences were observed in the number of non-fertilised oocytes (UFOs: 1.8±0.6 vs. 0.5±0.2; p=0.04) as well as in the number of degenerated embryos obtained (DE: 1.5±0.5 vs. 1.0±0.3; p= 0.02). However, no statistical differences were detected in the number of viable embryos obtained (9.2±2.6 vs. 4.38±1.2) when bscrFSH and FSH-p-derived protocols were compared (p= 0.08). In conclusion, the number of total structures, UFOs, and DE increased substantially per donor by using the bscrFSH-derived SOV protocol. Despite of the increase in the number of viable embryos obtained in Brangus heifers by using the bscrFSH-derived SOV protocol no differences were observed between groups. ANID 21201280.