Animal Reproduction (AR)
https://www.animal-reproduction.org/article/doi/10.21451/1984-3143-AR916
Animal Reproduction (AR)
Original Article

Effect of the type of egg yolk, removal of seminal plasma and donor age on ram sperm cryopreservation

Wilber García, Abigail Tabarez, Maria Jesus Palomo

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Abstract

This study was conducted to assess the effect on ram sperm freezability by the replacement of traditional fresh egg yolk (FEY) with fresh clarified egg yolk (CEY) or powdered egg yolk (PEY) in extenders. Simultaneously, the effect of semen washing and donor age (1 and 2 years old) was studied on thawed sperm quality. Briefly, in two consecutives autumns, ejaculates from 8 males were collected by artificial vagina, mixed and split into two samples. One sample was washed by centrifugation and the pellet was split into three aliquots and re-suspended in an extender containing 15% of different type of egg yolk (PEY, FEY or CEY) supplemented with 5% glycerol in a Tris-based medium. The other sample was directly split into three aliquots and diluted in the same extenders. All samples were chilled for 4 h at 5ºC before been frozen to −196ºC. The results suggested that powdered egg yolk can be used satisfactory on ram sperm cryopreservation ensuring greater bio-security meanwhile fresh clarified egg yolk did not improve sperm freezability. Moreover, semen from 2 years old donors was more resistant to cryopreservation than semen collected from younger males. Finally, sperm washing had a beneficial effect on sperm cryosurvival.

Keywords

age, cryopreservation, egg yolk, ram sperm, seminal plasma.

References

Aboagla EME, Terada T. 2003. Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biol Reprod, 69(4):1245-1250.

Anton M, Martinet V, Dalgalarrondo M, Beaumal V, David-Briand E, Rabesona, AH. 2003. Chemical and structural characterisation of low-density lipoproteins purified from hen egg yolk. Food Chem, 83:175-183.

Barrios B, Pérez-Pé R, Gallego M, Tato A, Osada J, Muino-Blanco T, Cebrián-Pérez JA. 2000. Seminal plasma proteins revert the cold-shock damage on ram sperm membrane. Biol Reprod, 1531-1537.

Barrios B, Fernandez-Juan M, Muiño-Blanco T, Cebrián-Pérez JA. 2005. Immunocytochemical localization and biochemical characterization of two seminal plasma proteins that protect ram spermatozoa against cold shock. J Androl, 26:539-549.

Bathgate R, Maxwell WM, Evans G. 2006. Studies on the effect of supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thawsperm quality. Reprod Domes Anim, 41:68-73.

Beltrán de Heredia I. 2009. Reproducción y control reproductivo en el macho. In Sañudo C, Cepero Briz R, (Eds). Ovinotecnia; Producción y Economía de la Especie. Zaragoza, Spain Prensas Universitarias de Zaragoza, pp.105-114.

Bergeron A, Crête MH, Brindle Y, Manjunath P. 2004. Low-density lipoprotein fraction from hen's egg yolk decreases the binding of the major proteins of bovine seminal plasma to sperm and prevents lipid efflux from the sperm membrane. Biol Reprod, 70(3):708-17.

Bispo CAS, Pugliesi G, Galvão P, Rodrigues MT, Ker P, Filgueiras B. 2011. Effect of low and high egg yolk concentrations in the semen extender for goat semen cryopreservation. Small Rumin Research, 100:54-58.

Bousseau S, Brillard JP, Marquant-Le Guienne B, Guerin B, Camus A, Lechat M. 1998. Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin based diluents. Theriogenology, 50:699-706.

Bravo J. 2010. Estudio de la influencia de la estación sobre los parámetros seminales y morfométricos del espermatozoide del morueco de la raza Ile de France. Tesis Facultad de Veterinaria. Universidad de Extremadura. Cáceres, España. 228p.

Cabrera F, González F, Batista M, Forga J, Calero P, Gracia A. 1998. Influencia de la edad y los factores ambientales sobre la producción seminal del macho de la agrupación caprina canaria (variedad Majorera) a lo largo del todo el año. XXIII Jornadas Científicas de la Sociedad Española de Ovinotecnia y Caprinotecnia.

Vitoria, Spain. p.525-528 Cebrián J, Muiño-Blanco T, Pérez-Pé R, Casao A. 2010. Manejo y conservación del semen. In Abecia A and Forcada F (Edts). Manejo reproductivo en ganado ovino. Servet, Zaragoza, Spain. pp:127-135.

Colas G, Zinsner F. 1975. Production spermatique et développement testiculaire chez l'agneau de race Ile-deFrance et Préalpes. Journées de Recherche Ovine et Caprine. Ed. ITOVIC, France, París, p.235-243.

Fernández-Santos MR, Esteso MC, Montoro V, Soler AJ, Garde JJ. 2006. Cryopreservation of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa:Effects of egg yolk, glycerol and cooling rate. Theriogenology, 66:1931-1942.

Folch J. 1984. Manejo reproductivo de los ovinos de carne y sus bases fisiológicas. Institución Fernando el Católico (Eds.). Zaragoza, España. p.43-46.

Forouzanfar M, Sharafi M, Hosseini SM, Ostdadhosseini S, Hajian M, Hosseini L, Abedi P, Nili N, Rahmani HR, Nasr-Esfahani MH. 2010. In vitro comparison of egg yolk–based and soybean lecithin–based extenders for cryopreservation of ram semen. Theriogenology, 73:480-487 Graham JK. 1994. Effect of seminal plasma on the motility of epididymal and ejaculated spermatozoa of the ram and bull during the cryopreservation process. Theriogenology, 41:1151-1162.

Hancock JL. 1951. A staining technique for the study of temperature shock in semen. Nature, 167:323-324.

Holt C, Holt WV, Moore HD. 1996. Choice of operating conditions to minimize sperm subpopulation sampling bias in the assessment of boar semen by computer-assisted semen analysis. J Androl, 17:587- 596.

Kampschmidt RF, Mayer DT, Heman HA. 1953. Lipid and lipoprotein constituents of egg yolk in the resistance and storage of bull spermatozoa. J Dairy Sci, 36:733-742.

Lymberopoulus AG, Tsakmakidis IA, Khalifa TAA. 2010. Effect of ram age on structural and functional competence of frozen–thawed spermatozoa in dairy sheep. Reprod Dom Anim, 45, 572-578 Marco-Jimenez F, Puchades S, Moce E, Viudes-DeCartro M, Vicente J, Rodriguez M. 2004. Use of Powdered Egg Yolk vs Fresh Egg Yolk for the Cryopreservation of Ovine Semen. Reprod Dom Anim, 39:438-441.

Martin JC, Klug E, Gunzel AR. 1979. Centrifugation jof stallion semen and its storage in large volume straws. Journal of Reproduction and Fertility, 27:47-51.

Martínez R, Vásquez R, Cerquera A, Espinosa E. 1998. Caracterización de la respuesta a criopreservación y evaluación por prueba de reacción acrosómica in vitro de la fertilidad del semen ovino. Rev Corpica Colombia, 11:90-97.

Maroto-Morales A. 2012. Evaluación objetiva de la morfometria de los espermatozoides de ovino (Ovis aries). Relacionados con la fertilidad. Facultad de Veterinaria, Universidad de Castilla-La Mancha, Spain. Thesis.

Maxwell WMC, Watson PF. 1996. Recent progress in the preservation of ram semen. Anim Reprod Sci, 42:55- 65.

Maxwell WMC, de Graaf SP, El-Hajj Ghaoui R, Evans G. 2007. Seminal plasma effects on sperm handling and fertility. In: Juengel JI, Murria JF and Smith MF (eds). Reproduction in Domestic Ruminants VI. Nottingham UK: Nottingham University Press, 13- 38.

Miranda J, Guerrero AF, Partal P. 2000. Reología de derivados de la yema de huevo deshidratada. Grasas y Aceites. 51:244-250.

Moussa M, Martinet V, Trimeche A, Tainturier D, Anton M. 2002. Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen thawed bull semen. Theriogenology, 57:1695-1706.

Muiño-Blanco T, Pérez-Pé R, Cebrián-Pérez JA. 2008. Seminal Plasma Proteins and Sperm Resistance to Stress. Reprod Dom Anim, 43(Suppl. 4):18-31.

Pace M, Graham P. 1974. Components in egg yolk which protect bovine spermatozoa during freezing. J Animal Science, 39(6):1144-1149.

Pérez-Pé R, Barrios B, Muiño-Blanco T, CebriánPérez JA. 2001. Seasonal differences in ram seminal plasma revealed by partition in anaqueous two-phase system. J Chrom B, 76:113-121.

Ritar AJ, Salamon S. 1991. Effects of month of collection, method of processing, concentration of egg yolk and duration of frozen storage on viability of Angora goat spermatozoa. Small Rumin Research, 4(1):29-37.

Rodríguez-Almeida F, Ávila CO, Anchondo A, Sánchez-Ramírez B, Jiménez JA. 2008. Capacitación espermática inducida por la conservación de semen de carnero diluido, refrigerado o congelado. AgrocienciaMexico. 42(4):399-406.

Salamon S, Maxwell WMC. 2000. Storage of ram semen. Anim Reprod Sci, 62:77-111.

Schmehl MK, Anderson SP, Vazquez IA, Graham EF. 1986. The effect of dialysis of extended ram semen prior to freezing on post-thaw survival and fertility. Cryobiology, 23:406-414.

Tabarez A, García W, Palomo MJ. 2017. Effect of the type of egg yolk, removal of seminal plasma and donor age on buck sperm cryopreservation. Small Rumin Res, 149:91-98.

Thibier M, Guerin B. 2000. Hygienic aspects of storage and use of semen for artificial insemination. Anim Reprod Sci, 62:233-251.

Wall RJ, Foote RH. 1999. Fertility of bull sperm frozen and stored in clarified egg yolk-Tris-glycerol extender. J Dairy Sci, 82:817-821.

Watson PF, Martin CA. 1975. The influence of some fractions of egg yolk on the survival of ram spermatozoa at 5o C. Aust J Biol Sci, 28:145-152.

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