Animal Reproduction (AR)
https://www.animal-reproduction.org/article/doi/10.21451/1984-3143-2018-0040
Animal Reproduction (AR)
Original Article

Partial ablation of endometrial glands in dogs after exposure to progestin during the neonatal period

Natália Soares Teixeira; Bianca Barcelos Martins; Rodrigo Volpato; José Leonardo Gualberto Ramos; Patricia Maria Coletto Freitas; Renée Laufer-Amorim; Maria Denise Lopes; Marcelo Rezende Luz

Downloads: 0
Views: 886

Abstract

Bitches with uteri devoid of endometrial glands should be sterile, and consequently could contribute to the population control of dogs. Considering that an inadequate exposure of the female reproductive system to steroids can lead to the formation of the uterine gland knock-out (UGKO) phenotype in some species, the aim of this study was to evaluate the effect of serial applications of medroxyprogesterone acetate (MPA) from birth until the age of six months on the development of endometrial glands in bitches. For this purpose, 16 female mongrel dogs from different litters were distributed into either an MPA group (n = 8), animals treated with 10 mg kg sc (Promone-E®, Pfizer, Brasil) at 3-week intervals, from day one after birth until the age of six months, or a control group (n = 8), composed of animals that only received a 0.9% NaCl solution in place of MPA. At six months of age, ovariohysterectomy was performed and uterine horn samples were collected for histological and immunohistochemical examinations. The bitches from the MPA-treated group presented a 35% decrease in the number of endometrial glands, a larger diameter of the endometrial glands, a greater epithelial height, as well as a greater thickness of the uterine wall, endometrium, and myometrium. However, no significant differences were observed between the two groups in the expression of ER-α, ER-β, and PR on the surface epithelium and endometrial stroma. Therefore, the serial application of MPA from birth until the age of 6 months do not completely ablate the development of the endometrial glands in bitches, but impair it by 35%.

Keywords

ablation, bitch, endometrial glands, receptors, uterus

References

Bartol FF , Johnson LL , Floyd JG , Wiley AA , Spencer TE , Buxton DF , Coleman DA . 1995 . Neonatal exposure to progesterone and estradiol alters uterine morphology and luminal protein content in adult beef heifers . Theriogenology , 43 : 835 - 844 .

Bartol FF , Wiley AA , Spencer TE , Ing NH , Ott TL , Bazer FW . 1997 . Progestin exposure from birth: epigenetic induction of a unique adult uterine phenotype in sheep a glandless endometrium . Biol Reprod , 56 : 133 . (abstract) .

Bazer FW . 1975 . Uterine protein secretions: relationship to development of the conceptus . J Anim Sci , 4 : 1376 - 1382 .

Bazer FW , Wu G , Johnson GA . 2017 . Pregnancy recognition in mammals: the roles of interferons and estrogens . Anim Reprod , 14 : 7 - 29 .

Bowen RA , Olson PN , Behrendt MD , Wheeler SL , Husted PW , Nett TM . 1985 . Efficacy and toxicity of estrogens commonly used to terminate canine pregnancy . J Am Vet Med Assoc , 15 : 783 - 788 .

Carson DD , Bagghi I , Dey SK , Enders AC , Fazleabas AT , Lessey BA , Yoshinaga K . 2000 . Embryo implantation . Dev Biol , 223 : 217 - 237 .

Carter AM , Mess AM . 2017 . The evolution of fetal membranes and placentation in carnivores and ungulates (Ferungulata) . Anim Reprod , 14 : 124 - 135 .

Clarke IJ , Horton RJE , Doughton BW . 1990 . Investigation of the mechanism by which insulin-induced hypoglycemia decreases luteinizing hormone secretion in ovariectomized ewes . Endocrinology , 127 : 1470 - 1476 .

Concannon PW , Spraker TR , Casey HW , Hansel W . 1981 . Gross and histopathologic effects of medroxyprogesterone acetate and progesterone on the mammary glands of adult beagle bitches . Fertil Steril , 36 : 373 - 387 .

Cooke PS , Borsdorf DC , Ekman GC , Doty KF , Clark SG , Dziuk PJ , Bartol FF . 2012 . Uterine gland development begins postnatally and is accompanied by estrogen and progesterone receptor expression in the dog . Theriogenology , 78 : 1787 - 1795 .

Cooper RL , Kavlock RJ . 1997 . Endocrine disruptors and reproductive development: a weight-of-evidence overview . J Endocrinol , 152 : 159 - 166 .

De Bosschere H , Ducatelle R , Tshamala M , Coryn M. 2002 . Changes in sex hormone receptors during administration of progesterone to prevent estrus in the bitch . Theriogenology , 6 : 1209 - 1217 .

Dhaliwal GK , England GC , Noakes De W . 1999 . The influence of exogenous steroid hormones on steroid receptors, uterine histological structure and the bacterial flora of the normal bitch . Anim Reprod Sci , 56 : 259 - 277 .

Galabova GK , Walter I , Aurich C , Aurich JE . 2004 . Steroid receptors in canine endometrial cells can be regulated by estrogen and progesterone under in vitro conditions . Theriogenology , 61 : 963 - 976 .

Gerstenberg C , Allen WR . 1999 . Development of the equine endometrial glands from fetal life to ovarian cycling . J Reprod Fertil , 56 : 317 - 326 .

Gobello C , Castex G , Klima L , Rodríguez R , Corrada Y. 2003 . Study of two protocol combining aglepristone and cloprostenol to treat open cervix pyometra in the bitch . Theriogenology , 15 : 901 - 908 .

Gray CA , Bartol FF , Tarleton BJ , Wiley AA , Johnson GA , Bazer FW , Spencer TE . 2001 . Developmental biology of uterine glands . Biol Reprod , 65 : 1311 - 1323 .

Howe LM . 2006 . Surgical methods of contraception and sterilization . Theriogenology , 66 : 500 - 509 .

Lessey BA , Gorell TA . 1981 . Nuclear progesterone receptors in the beagle uterus . J Steroid Biochem , 14 : 585 - 591 .

Maenhoudt C , Santos NR , Fontbonne A . 2014 . Supression of fertility in adult dogs . Reprod Domest Anim , 49 : 58 - 63 .

Muulac-Jericevic B , Conneely OM . 2004 . Reproductive tissue selective actions of progesterone receptors . Reproduction , 128 : 139 - 146 .

Okulicz WC . 1986 . Progesterone receptor replenishment during sustained progesterone treatment in the hamster uterus . Endocrinology , 118 : 2488 - 2494 .

Perry JS , Crombie PR . 1982 . Ultrastructure of the uterine glands of the pig . J Anat , 134 : 339 - 350 .

Ponchon T , Merlo ML , Faya M , Priotto M , Barbeito C , Gobello C . 2015 . Postnatal exposure to a progestin does not prevent uterine adenogenesis in domestic dogs . J Vet Sci , 17 : 111 - 113 .

Ramos JLG , Ramos CLFG , Cunha ICN , Carvalho ECQ , Shimoda E , Luz MR . 2015 . Análise histomorfométrica do útero na espécie canina do nascimento aos seis meses de idade . Arq Bras Med Vet Zootec , 67 : 41 - 48 .

Roberts RM , Bazer FW . 1988 . The function of uterine secretions . J Reprod Fertil , 82 : 875 - 892 .

Romagnoli S , Concannon PW . 2003 . Clinical use of progestins in bitches and queens: a review. In: Concannon PW , England G , Verstegen J , Linde-Forsberg C (Ed.). Recent Advances in Small Animal Reproduction. New York, NY : International Veterinary Service, University of Cornell .

Santos FC , Corrêa TP , Rahal SC , Crespilho AM , Lopes , MD , Mamprim MJ . 2009 . Complicações da esterilização cirúrgica de fêmeas caninas e felinas. Revisão de literatura . Vet Zootec , 16 : 8 - 18 .

Selman PJ , Van Garderen E , Mool JA , Van Den Ingh TS . 1995 . Comparison of the histological changes in the dog after treatment with the progestins medroxyprogesterone acetate and proligestone . Vet Q , 17 : 128 - 133 .

Stewart T , Sage R , Stewart A , Cameron D. 2000 . Breast cancer incidence highest in the range of one species of house mouse, Mus domesticus. Br J Cancer , 82 : 446 - 451 .

Volpato R. 2011 . Avaliação clínica e imunoistoquímica do útero e cérvix de cadelas com o diagnóstico de piometra . Botucatu, SP , Universidade Estadual Paulista . 28 pp. Dissertation (MS) .

Vermeirsch H , Simoens P , Hellemans A , Coryn M , Lauwers H. 2000 . Immunohistochemical detection of progesterone receptors in the canine uterus and their relation to sex steroid hormone levels . Theriogenology , 53 : 773 - 788 .

Vermeirsch H , Simoens P , Lauwers H , Coryn M. 1999 . Immunohistochemical detection of estrogen receptors in the canine uterus and their relation to sex steroid hormone levels . Theriogenology , 51 : 729 - 43 .

West NB , Verhage HG , Brenner RM . 1976 . Suppression of the estradiol receptor system by progesterone in the oviduct and uterus of the cat . Endocrinology , 99 : 1010 - 1016 .

West NB , Verhage HG , Brenner RM . 1977 . Changes in nuclear estradiol receptor and cell structure during estrous cycles and pregnancy in the oviduct and uterus of cats . Biol Reprod , 17 : 138 - 143 .

Wilsher S , Lefranc AC , Allen WR . 2009 . Post natal oestrogen administration stimulates precocious endometrial gland development in the horse . Equine Vet J , 41 : 678 - 684 .

 

 

 

5b7571a00e882503488068a8 animreprod Articles
Links & Downloads

Anim Reprod

Share this page
Page Sections