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Comizzoli P, Mauget R, Mermillod P. Assessment of in vitro fertility of deer spermatozoa by heterologous IVF with zona-free bovine oocytes. Theriogenology. 2001;56(2):261-74. https://doi.org/10.1016/S0093-691X(01)00561-1. PMid:11480618.

Comizzoli P, Power ML, Bornbusch SL, Muletz-Wolz CR. Interactions between reproductive biology and microbiomes in wild animal species. Anim Microbiome. 2021;3(1):87. https://doi.org/10.1186/s42523-021-00156-7. PMid:34949226.

Comizzoli P, Pukazhenthi BS, Wildt DE. The competence of germinal vesicle oocytes is unrelated to nuclear chromatin configuration and strictly depends on cytoplasmic quantity and quality in the cat model. Hum Reprod. 2011;26(8):2165-77. https://doi.org/10.1093/humrep/der176. PMid:21665874.

Comizzoli P, Songsasen N, Hagedorn M, Wildt DEE. Comparative cryobiological traits and requirements for gametes and gonadal tissues collected from wildlife species. Theriogenology. 2012;78(8):1666-81. https://doi.org/10.1016/j.theriogenology.2012.04.008. PMid:22704386.

Comizzoli P, Urner F, Sakkas D, Renard JPP. Up-regulation of glucose metabolism during male pronucleus formation determines the early onset of the s phase in bovine zygotes. Biol Reprod. 2003;68(5):1934-40. https://doi.org/10.1095/biolreprod.102.011452. PMid:12606355.

Comizzoli P, Wildt DE, Pukazhenthi BS. In vitro compaction of germinal vesicle chromatin is beneficial to survival of vitrified cat oocytes. Reprod Domest Anim. 2009;44(s2 Suppl 2):269-74. https://doi.org/10.1111/j.1439-0531.2009.01372.x. PMid:19754584.

Comizzoli P, Wildt DE, Pukazhenthi BS. Poor centrosomal function of cat testicular spermatozoa impairs embryo development in vitro after intracytoplasmic sperm injection. Biol Reprod. 2006;75(2):252-60. https://doi.org/10.1095/biolreprod.106.051342. PMid:16687647.

Comizzoli P, Wildt DE, Pukazhenthi BS. Effect of 1,2-propanediol versus 1,2-ethanediol on subsequent oocyte maturation, spindle integrity, fertilization, and embryo development in vitro in the domestic cat. Biol Reprod. 2004;71(2):598-604. https://doi.org/10.1095/biolreprod.104.027920. PMid:15084479.

Comizzoli P, Wildt DEDE, Pukazhenthi BSBS. Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat. Mol Reprod Dev. 2008;75(2):345-54. https://doi.org/10.1002/mrd.20769. PMid:17701993.

Cowl VB, Comizzoli P, Appeltant R, Bolton RL, Browne R, Holt WV, Penfold LM, Swegen A, Walker SL, Williams SA. Cloning for the twenty-first century and its place in endangered species conservation. Annu Rev Anim Biosci. 2023;12(1):91-112. https://doi.org/10.1146/annurev-animal-071423-093523. PMid:37988633.

Crosier AE, Lamy J, Bapodra P, Rapp S, Maly M, Junge R, Haefele H, Ahistus J, Santiestevan J, Comizzoli P. First birth of cheetah cubs from in vitro fertilization and embryo transfer. Animals (Basel). 2020;10(10):1811. https://doi.org/10.3390/ani10101811. PMid:33027972.

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Elliott GD, Lee P-C, Paramore E, Van Vorst M, Comizzoli P. Resilience of oocyte germinal vesicles to microwave-assisted drying in the domestic cat model. Biopreserv Biobank. 2015;13(3):164-71. https://doi.org/10.1089/bio.2014.0078. PMid:26035005.

Holt WV, Comizzoli P. Reproductive science and wildlife conservation: the knowns and unknowns! Reprod Fertil. 2026;7(1):250135. https://doi.org/10.1530/RAF-25-0135. PMid:41746758.

Howard JG, Lynch C, Santymire RM, Marinari PE, Wildt DE. Recovery of gene diversity using long-term cryopreserved spermatozoa and artificial insemination in the endangered black-footed ferret. Anim Conserv. 2016;19(2):102-11. https://doi.org/10.1111/acv.12229.

Hutchinson AM, Appeltant R, Burdon T, Bao Q, Bargaje R, Bodnar A, Chambers S, Comizzoli P, Cook L, Endo Y, Harman B, Hayashi K, Hildebrandt T, Korody ML, Lakshmipathy U, Loring JF, Munger C, Ng AHM, Novak B, Onuma M, Ord S, Paris M, Pask AJ, Pelegri F, Pera M, Phelan R, Rosental B, Ryder OA, Sukparangsi W, Sullivan G, Tay NL, Traylor-Knowles N, Walker S, Weberling A, Whitworth DJ, Williams SA, Wojtusik J, Wu J, Ying QL, Zwaka TP, Kohler TN. Advancing stem cell technologies for conservation of wildlife biodiversity. Development. 2024;151(20):dev203116. https://doi.org/10.1242/dev.203116. PMid:39382939.

Lee P-C, Adams DM, Amelkina O, White KK, Amoretti LA, Whitaker MG, Comizzoli P. Influence of microwave-assisted dehydration on morphological integrity and viability of cat ovarian tissues: first steps toward long-term preservation of complex biomaterials at supra-zero temperatures. PLoS One. 2019;14(12):e0225440. https://doi.org/10.1371/journal.pone.0225440. PMid:31800613.

Lee PC, Comizzoli P. Desiccation and supra-zero temperature storage of cat germinal vesicles lead to less structural damage and similar epigenetic alterations compared to cryopreservation. Mol Reprod Dev. 2019;86(12):1822-31. https://doi.org/10.1002/mrd.23276. PMid:31549479.

Lee PC, Stewart S, Amelkina O, Sylvester H, He X, Comizzoli P. Trehalose delivered by cold-responsive nanoparticles improves tolerance of cumulus-oocyte complexes to microwave drying. J Assist Reprod Genet. 2023;40(8):1817-28. https://doi.org/10.1007/s10815-023-02831-x. PMid:37261586.

Lee PC, Zahmel J, Jewgenow K, Comizzoli P. Desiccated cat spermatozoa retain DNA integrity and developmental potential after prolonged storage and shipping at non-cryogenic temperatures. J Assist Reprod Genet. 2022;39(1):141-51. https://doi.org/10.1007/s10815-021-02337-4. PMid:34609666.

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Martin-Wintle MS, Kersey DC, Wintle NJP, Aitken-Palmer C, Owen MA, Swaisgood RR. Comprehensive breeding techniques for the giant panda. In: Comizzoli P, Brown J, Holt W, editors. Reproductive sciences in animal conservation. Cham: Springer; 2019. p. 275–308. https://doi.org/10.1007/978-3-030-23633-5_10.

Mastromonaco G. 40 ‘wild’years: the current reality and future potential of assisted reproductive technologies in wildlife species. Anim Reprod. 2024;21(3):e20240049. https://doi.org/10.1590/1984-3143-ar2024-0049. PMid:39286364.

Mastromonaco G, Comizzoli P. Back to basics. Theriogenology. 2018;109:1. https://doi.org/10.1016/j.theriogenology.2018.01.005. PMid:30853060.

Morrow CJ, Penfold LM, Wolfe BA. Artificial insemination in deer and non-domestic bovids. Theriogenology. 2009;71(1):149-65. https://doi.org/10.1016/j.theriogenology.2008.09.001. PMid:18986694.

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Pukazhenthi B, Comizzoli P, Travis AJAJ, Wildt DEDE. Applications of emerging technologies to the study and conservation of threatened and endangered species. Reprod Fertil Dev. 2006;18(1-2):77-90. PMid:16478605.

Pukazhenthi BS, Wildt DE. Which reproductive technologies are most relevant to studying, managing and conserving wildlife? Reprod Fertil Dev. 2004;16(1-2):33-46. https://doi.org/10.1071/RD03076. PMid:14972101.

Silva AR, Bezerra LGP, Dos Santos RP, Moreira SSJ, Lima GL, Comizzoli P. The Tayassuids. In: Silva AR, editor. Assisted reproduction in wild mammals of South America. Boca Raton: CRC Press; 2023. p. 29–47. https://doi.org/10.1201/9781003231691-4.

Swanson WF. The challenge of assisted reproduction for conservation of wild felids-A reality check. Theriogenology. 2023;197:133-8. https://doi.org/10.1016/j.theriogenology.2022.11.018. PMid:36502591.

Thongphakdee A, Berg DK, Tharasanit T, Thongtip N, Tipkantha W, Punkong C, Tongthainan D, Noimoon S, Maikeaw U, Kajornklin N, Siriaroonrat B, Comizzoli P, Kamolnorranath S. The impact of ovarian stimulation protocol on oocyte quality, subsequent in vitro embryo development, and pregnancy after transfer to recipients in Eld’s deer (Rucervus eldii thamin). Theriogenology. 2017;91:134-44. https://doi.org/10.1016/j.theriogenology.2016.12.021. PMid:28215678.

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