Animal Reproduction (AR)
https://www.animal-reproduction.org/article/doi/10.1590/1984-3143-AR2025-0013
Animal Reproduction (AR)
ORIGINAL ARTICLE

Establishment of a mouse embryo bank at ICTB/FIOCRUZ: vitrification of genetically modified strains

Isabella de Moura Folhadella Pires; Janaína Barcelos Porto Ferreira; Luciene Paschoal Braga Dias; Cristiano Magalhães Ferreira Sobrinho; Alessandra de Almeida Ramos; Paulo César da Silva Souza; Thaís Malheiros Torres; Fabiana Batalha Knackfuss

http://dx.doi.org/10.1590/1984-3143-AR2025-0013 Anim Reprod, vol.22, n4, e20250013, 2025
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Abstract

To establish a mouse embryo bank at the Institute of Science and Technology in Biomodels, Oswaldo Cruz Foundation (ICTB/FIOCRUZ), embryos from genetically modified strains were vitrified. The strains included B6.129SVEV-CCBP2 (D6), B6.129P2-Nos2 (Nos2), B6.129S2-Cd28 (Cd28), B6.129P2-Ccl3 (Ccl3), B6.129S2-Alox5 (Alox5), B6.129P2-Ccr2 (Ccr2), B6.129P2-Ccr5 (Ccr5) and B6.129S1-Tlr6 (Tlr6). To accomplish this, the animals were superovulated and mated, and their embryos were collected and vitrified. The success of the technique was evaluated by examining the development of the embryos through thawing and in vitro culture, comparing them to a control group. The results were analyzed using percentages, Tukey's t-test, and Analysis of Variance. The embryonic development percentages for the different strains were as follows: D6 (55%), Nos2 (24.7%), Cd28 (45.8%), Ccl3 (50%), Alox5 (4.8%), Ccr2 (66.7%), Ccr5 (63.04%) and Tlr6 (52.8%). Significant differences were observed between the strains Nos2 (p=0.0434), Cd28 (p=0.034), Ccl3 (p=0.0006), and Alox5 (p=0.0166) compared to their respective control groups. In conclusion, the strains Ccr2 (p= p=0.0889), Ccr5 (p=0.0806), D6(p=0,0685) and Tlr6 (p=0.0806) demonstrated favorable results in terms of the vitrification protocol and subsequent embryonic development, as they did not significantly differ from the control groups.

Keywords

mice, vitrification, transgenic, embryos

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Submitted date:
02/06/2025

Accepted date:
07/11/2025

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