Animal Reproduction (AR)
https://www.animal-reproduction.org/article/doi/10.1590/1984-3143-AR2023-0155
Animal Reproduction (AR)
ORIGINAL ARTICLE

Immunomodulatory-associated gene transcripts to multipotency of bovine amniotic fluid mesenchymal stem cells

Jamila Cristina Baptistella; Carolina Gonzales da Silva; Sônia Nair Báo; Letícia Colin Panegossi; Tereza Cristina Cardoso; Roberto Gameiro de Carvalho; Carlos Frederico Martins

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Abstract

The adnexa fetal tissues are sources of mesenchymal stromal cells (MSCs) due to their noninvasive harvest, with all biological material discarded most of the time. MSCs are a promise regarding to their plasticity, self-renewal, differentiation potentials, immunomodulatory and anti-inflammatory properties, which have made clinical stem cell therapy a reality. The present study aimed to characterize and evaluate the immunomodulation ability of bovine mesenchymal cells collected from bovine amniotic fluid (bAFMSCs) isolated and subjected to sixth consecutive culture passages in vitro. The multilineage properties of the bAFMSCs collections confirmed the ability to undergo adipogenic, chondrogenic and osteogenic differentiation. The mesenchymal gene transcription CD106, CD73, CD29, CD90 and CD166 were detected in bAFMSCs, whereas CD34 and CD45 were not detected. Regarding cytokine mRNA expression, IL2, IL6, INFα, INFβ, INFγ, TNFα and TNFβ were downregulated, while IL10 was highly regulated in all studied passages. The present study demonstrated the immunological properties and multipotency of in vitro bAFMSCs collections, and thus, they can be tested in cattle pathological treatments or multiplication by nuclear transfer cloning.

Keywords

bovine mesenchymal stromal cells, differentiation, immunomodulation, gene expression

References

Ankrum JA, Ong JF, Karp JM. Mesenchymal stem cells: immune evasive, not immune privileged. Nat Biotechnol. 2014;32(3):252-60. http://dx.doi.org/10.1038/nbt.2816. PMid:24561556.

Carrade DD, Borjesson DL. Immunomodulation by mesenchymal stem cells in veterinary species. Comp Med. 2013;63(3):207-17. PMid:23759523.

Corradetti B, Meucci A, Bizzaro D, Cremonesi F, Lange Consiglio A. Mesenchymal stem cells from amnion and amniotic fluid in the bovine. Reproduction. 2013;145(4):391-400. http://dx.doi.org/10.1530/REP-12-0437. PMid:23404849.

Cunha ER, Martins CF, Silva CG, Bessler HC, Báo SN. Effects of prolonged in vitro culture and cryopreservation on viability, DNA fragmentation, chromosome stability and ultrastructure of bovine cells from amniotic fluid and umbilical cord. Reprod Domest Anim. 2014;49(5):806-12. http://dx.doi.org/10.1111/rda.12372. PMid:25131149.

Di Trapani M, Bassi G, Ricciardi M, Fontana E, Bifari F, Pacelli L, Giacomello L, Pozzobon M, Féron F, De Coppi P, Anversa P, Fumagalli G, Decimo I, Menard C, Tarte K, Krampera M. Comparative study of immune regulatory properties of stem cells derived from different tissues. Stem Cells Dev. 2013;22(22):2990-3002. http://dx.doi.org/10.1089/scd.2013.0204. PMid:23819720.

Huaman O, Bahamonde J, Cahuascanco B, Jervis M, Palomino J, Torres CG, Peralta OA. Immunomodulatory and immunogenic properties of mesenchymal stem cells derived from bovine fetal bone marrow and adipose tissue. Res Vet Sci. 2019;124(1):212-22. http://dx.doi.org/10.1016/j.rvsc.2019.03.017. PMid:30925336.

Kang JW, Kang KS, Koo HC, Park JR, Choi EW, Park YH. Soluble factors-mediated immunomodulatory effects of canine adipose tissue-derived mesenchymal stem cells. Stem Cells Dev. 2008;17(4):681-94. http://dx.doi.org/10.1089/scd.2007.0153. PMid:18717642.

Kode JA, Mukherjee S, Joglekar MV, Hardikar AA. Mesenchymal stem cells: immunobiology and role in immunomodulation and tissue regeneration. Cytotherapy. 2009;11(4):377-91. http://dx.doi.org/10.1080/14653240903080367. PMid:19568970.

Miguel MP, Fuentes-Julián S, Blázquez-Martínez A, Pascual CY, Aller MA, Arias J, Arnalich-Montiel F. Immunosuppressive properties of mesenchymal stem cells: advances and applications. Curr Mol Med. 2012;12(5):574-91. http://dx.doi.org/10.2174/156652412800619950. PMid:22515979.

Mukonoweshuro B, Brown CJ, Fisher J, Ingham E. Immunogenicity of undifferentiated and differentiated allogeneic mouse mesenchymal stem cells. J Tissue Eng. 2014;5(1):2041731414534255. http://dx.doi.org/10.1177/2041731414534255. PMid:24812582.

Peroni JF, Borjesson DL. Anti-inflammatory and immunomodulatory activities of stem cells. Vet Clin North Am Equine Pract. 2011;27(2):351-62. http://dx.doi.org/10.1016/j.cveq.2011.06.003. PMid:21872763.

Pieri NCG, de Souza AF, Botigelli RC, Machado LS, Ambrosio CE, Dos Santos Martins D, de Andrade AFC, Meirelles FV, Hyttel P, Bressan FF. Stem cells on regenerative and reproductive science in domestic animals. Vet Res Commun. 2019;43(1):7-16. http://dx.doi.org/10.1007/s11259-019-9744-6. PMid:30656543.

Poncelet AJ, Vercruysse J, Saliez A, Gianello P. Although pig allogeneic mesenchymal stem cells are not immunogenic in vitro, intracardiac injection elicits an immune response in vivo. Transplantation. 2007;83(6):783-90. http://dx.doi.org/10.1097/01.tp.0000258649.23081.a3. PMid:17414713.

Prasanna SJ, Gopalakrishnan D, Shankar SR, Vasandan AB. Pro-inflammatory cytokines, IFNgamma and TNFalpha, influence immune properties of human bone marrow and Wharton jelly mesenchymal stem cells differentially. PLoS One. 2010;5(2):e9016. http://dx.doi.org/10.1371/journal.pone.0009016. PMid:20126406.

Raoufi M, Tajik P, Dehghan MM, Eini F, Barin A. A. Isolation and differentiation of mesenchymal stem cells from bovine umbilical cord blood. Reprod Domest Anim. 2011;46(1):95-9. http://dx.doi.org/10.1111/j.1439-0531.2010.01594.x. PMid:20345587.

Saulnier N, Loriau J, Febre M, Robert C, Rakic R, Bonte T, Buff S, Maddens S. Canine placenta: A promising potential source of highly proliferative and immunomodulatory mesenchymal stromal cells? Vet Immunol Immunopathol. 2016;171(1):47-55. http://dx.doi.org/10.1016/j.vetimm.2016.02.005. PMid:26964717.

Sharma N, Jeong DK. Stem cell research: a novel boulevard towards improved bovine mastitis management. Int J Biol Sci. 2013;9(8):818-29. http://dx.doi.org/10.7150/ijbs.6901. PMid:23983615.

Silva CG, Martins CF, Cardoso TC, Cunha ER, Bessler HC, Martins GHL, Pivato I, Báo SN. Production of bovine embryos and calves cloned by nuclear transfer using mesenchymal stem cells from amniotic fluid and adipose tissue. Cell Reprogram. 2016;18(2):127-36. http://dx.doi.org/10.1089/cell.2015.0064. PMid:27055630.

Somal A, Bhat IA, Pandey S, Ansari MM, Indu B, Panda BSK, Bharti MK, Chandra V, Saikumar G, Sharma GT. Comparative analysis of the immunomodulatory potential of caprine fetal adnexa derived mesenchymal stem cells. Mol Biol Rep. 2021;48(5):3913-23. http://dx.doi.org/10.1007/s11033-021-06383-0. PMid:34050503.

Tessier L, Bienzle D, Williams LB, Koch TG. Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells. PLoS One. 2015;10(4):e0122954. http://dx.doi.org/10.1371/journal.pone.0122954. PMid:25902064.

Troyer DL, Weiss ML. Wharton’s jelly-derived cells are a primitive stromal cell population. Stem Cells. 2008;26(3):591-9. http://dx.doi.org/10.1634/stemcells.2007-0439. PMid:18065397.

Weiss ML, Anderson C, Medicetty S, Seshareddy KB, Weiss RJ, VanderWerff I, Troyer D, McIntosh KR. Immune properties of human umbilical cord Wharton’s jelly-derived cells. Stem Cells. 2008;26(11):2865-74. http://dx.doi.org/10.1634/stemcells.2007-1028. PMid:18703664.
 


Submitted date:
12/04/2023

Accepted date:
03/04/2024

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