Animal Reproduction (AR)
Animal Reproduction (AR)

Effect of the presence and location of corpus luteum on competence of bovine cumulus-oocyte complexes

Edgar Ricardo Moreno Jerez; Alejandra Arias García; Marina Caccia; Aldemar Chávez Rodríguez; Silvia Juliana Restrepo Gonzales; Edgar Mauricio Mogollón Waltero; Diego Fernando Dubeibe Marín

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Abstract: This study aimed to determine the effect of presence of the corpus luteum (CL) and its influence on cumulus–oocyte complexes (COCs) obtained from the ipsilateral or contralateral ovary in bovine on the recovery and capacity of the oocytes to sustain mono-spermic fertilization, undergo preimplantation development, and develop to the blastocyst stage. Ovaries were collected at a local slaughterhouse and kept in pairs corresponding to the same animal. In the first experiment the variables evaluated were compared between cows with (CCL+) and without (CCL-) CL, and for the second experiment, comparisons were made between ovaries with an ipsilateral (CL+), contralateral (CL), and no (NCL). The recovery rate of COCs was higher in ovaries from CCL cows, and a higher proportion of grade 1 COCs were recovered from this group. A higher proportion of metaphase I oocytes at 7 h of maturation, and a higher rate of cleavage were observed in the CCL+ group; however, a higher proportion of embryos were obtained from the CCL group. Besides, COCs from the CL+ group had a lower proportion of grades 1 and 2 morphological qualities, lower rate of metaphase II oocytes at 22 h of maturation, and lower rate of formation of two pronuclei, whereas a higher proportion of unfertilized oocytes after in vitro fertilization. On the other hand, the COCs from the CL group displayed a lower proportion of oocytes with more than two pronuclei, higher cleavage rate, and higher final blastocyst production were obtained when compared to CL+. Thus, the effects of CL on the competence of bovine COCs are different depending on the anatomical proximity of their location in the animal, negatively affecting the quality of COCs located in the same ovary, but not having negative effects on the competence of COCs in the ovaries contralateral to their location.


oocyte competence, sperm penetration, nuclear maturation, bovine embryos


Argudo DE, Tenemaza MA, Merchán SL, Balvoa JA, Méndez MS, Soria ME, Galarza LR, Ayala LE, Hernández-Fonseca HJ, Perea MS, Perea FP. Intraovarian influence of bovine corpus luteum on oocyte morphometry and developmental competence, embryo production and cryotolerance. Theriogenology. 2020;155:232-9. PMid:32758994.

Baruselli PS, Vieira LM, Batista EOS, Ferreira RM, Sales JNS, Gimenes LU, Torres-Junior JRS, Martins CM, Sá MF Fo, Bo GA. Updates on embryo production strategies. Anim Reprod. 2015;12(3):375-82.

Bisinotto RS, Santos JEP. The use of endocrine treatments to improve pregnancy rates in cattle. Reprod Fertil Dev. 2011;24(1):258-66. PMid:22394967.

Diaz T, Schmitt EJ, de la Sota RL, Thatcher MJ, Thatcher WW. Human chorionic gonadotropin-induced alterations in ovarian follicular dynamics during the estrous cycle of heifers. J Anim Sci. 1998;76(7):1929-36. PMid:9690649.

Diskin MG, Mackey DR, Roche JF, Sreenan JM. Effects of nutrition and metabolic status on circulating hormones and ovarian follicle development in cattle. Anim Reprod Sci. 2003;78(3-4):345-70. PMid:12818653.

Fair T, Lonergan P. The role of progesterone in oocyte acquisition of developmental competence. Reprod Domest Anim. 2012;47(4, Suppl. 4):142-7. PMid:22827363.

Ferré LB, Kjelland ME, Strøbech LB, Hyttel P, Mermillod P, Ross PJ. Review: recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods. Animal. 2020;14(5):991-1004. PMid:31760966.

Ginther OJ, Dangudubiyyam SV. Factors affecting side of ovulation in heifers and mares: a comparative study. Anim Reprod Sci. 2018;199:72-8. PMid:30447890.

Ginther OJ. Intraovarianism: local mechanisms that affect follicle and luteal dynamics in heifers and women. Biol Reprod. 2020;102(2):265-75. PMid:31621836.

Gonzalez-Bulnes A, Berlinguer F, Cocero MJ, Garcia-Garcia RM, Leoni G, Naitana S, Rosati I, Succu S, Veiga-Lopez A. Induction of the presence of corpus luteum during superovulatory treatments enhances in vivo and in vitro blastocysts output in sheep. Theriogenology. 2005;64(6):1392-403. PMid:16139615.

Guerreiro BM, Batista EOS, Vieira LM, Sá Filho MF, Rodrigues CA, Castro A No, Silveira CRA, Bayeux BM, Dias EAR, Monteiro FM, Accorsi M, Lopes RNVR, Baruselli PS. Plasma anti-mullerian hormone: an endocrine marker for in vitro embryo production from Bos taurus and Bos indicus donors. Domest Anim Endocrinol. 2014;49:96-104. PMid:25136816.

Hajarian H, Shahsavari MH, Karami-shabankareh H, Dashtizad M. The presence of corpus luteum may have a negative impact on in vitro developmental competency of bovine oocytes. Reprod Biol. 2016;16(1):47-52. PMid:26952753.

Karami HK, Shahsavari MH, Hajarian H, Moghaddam G. In vitro developmental competence of bovine oocytes: effect of corpus luteum and follicle size. Iran J Reprod Med. 2015;13(10):615-22. PMid:26644789.

Leibfried L, First NL. Characterization of bovine follicular oocytes and their ability to mature in vitro. J Anim Sci. 1979;48(1):76-86. PMid:573253.

Lonergan P, Fair T. In vitro-produced bovine embryos: dealing with the warts. Theriogenology. 2008;69(1):17-22. PMid:17950823.

Lonergan P, Fair T. Maturation of oocytes in vitro. Annu Rev Anim Biosci. 2016;4(1):255-68. PMid:26566159.

Lonergan P, Sánchez JM. Symposium review: progesterone effects on early embryo development in cattle. J Dairy Sci. 2020;103(9):8698-707. PMid:32622590.

Machatková M, Jokešová E, Petelíková J, Dvořáček V. Developmental competence of bovine embryos derived from oocytes collected at various stages of the estrous cycle. Theriogenology. 1996;45(4):801-10. PMid:16727842.

Manjunatha BM, Gupta PSP, Ravindra JP, Devaraj M, Ramesh HS, Nandi S. In vitro developmental competence of buffalo oocytes collected at various stages of the estrous cycle. Theriogenology. 2007;68(6):882-8. PMid:17706758.

Matás C, Vieira L, García-Vázquez FA, Avilés-López K, López-Úbeda R, Carvajal JA, Gadea J. Effects of centrifugation through three different discontinuous Percoll gradients on boar sperm function. Anim Reprod Sci. 2011;127(1-2):62-72. PMid:21784589.

Merton JS, de Roos APW, Mullaart E, de Ruigh L, Kaal L, Vos PLAM, Dieleman SJ. Factors affecting oocyte quality and quantity in commercial application of embryo technologies in the cattle breeding industry. Theriogenology. 2003;59(2):651-74. PMid:12499010.

Moreno JF, Flores-Foxworth G, Westhusin M, Kraemer DC. Influence of pregnancy and presence of a CL on quantity and quality of bovine oocytes obtained from ovarian follicles aspirated post-mortem. Theriogenology. 1993;39(1):271.

Nasser LF, Sá Filho MF, Reis EL, Rezende CR, Mapletoft RJ, Bó GA, Baruselli PS. Exogenous progesterone enhances ova and embryo quality following superstimulation of the first follicular wave in Nelore (Bos indicus) donors. Theriogenology. 2011;76(2):320-7. PMid:21496903.

Penitente-Filho J, Carrascal E, Oliveira F, Zolini A, Oliveira C, Soares Í, Torres C. Influence of dominant follicle and corpus luteum on recovery of good quality oocytes for in vitro embryo production in cattle. Br Biotechnol J. 2014;4(12):1305-12.

Penitente-Filho JM, Jimenez CR, Zolini AM, Carrascal E, Azevedo JL, Silveira CO, Oliveira FA, Torres CAA. Influence of corpus luteum and ovarian volume on the number and quality of bovine oocytes. Anim Sci J. 2015;86(2):148-52. PMid:25187422.

Pirestani A, Hosseini SM, Hajian M, Forouzanfar M, Moulavi F, Abedi P, Gourabi H, Shahverdi A, Taqi Dizaj AV, Nasr Esfahani MH. Effect of ovarian cyclic status on in vitro embryo production in cattle. Int J Fertil Steril. 2011;4(4):172-5. PMid:24851178.

Prentice-Biensch JR, Singh J, Alfoteisy B, Anzar M. A simple and high-throughput method to assess maturation status of bovine oocytes: comparison of anti-lamin A/C-DAPI with an aceto-orcein staining technique. Theriogenology. 2012;78(7):1633-8. PMid:22980092.

Quezada-Casasola A, Martínez-Armendáriz KE, Itzá-Ortiz MF, Escárcega-Ávila AM, Pérez-Eguía E, Filipiak Y, Larocca C, Carrera-Chávez JM. Effect of presence of corpora lutea on cumulus expansion of in vitro matured bovine oocytes selected by trypan blue and brilliant cresyl blue tests. J Appl Anim Res. 2018;46(1):967-72.

Saad M, Sarwar Z, Saleem M, Arshad U, Shahzad M, Hassan Mushtaq M, Husnain A, Riaz A, Ahmad N. Effect of plasma progesterone on oocyte recovery, oocyte quality, and early in-vitro developmental competence of embryos in Bos indicus dairy cows. Anim Reprod Sci. 2019;202:80-6. PMid:30765111.

Sales JNS, Iguma LT, Batista RITP, Quintão CCR, Gama MAS, Freitas C, Pereira MM, Camargo LSA, Viana JHM, Souza JC, Baruselli PS. Effects of a high-energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos taurus cows. J Dairy Sci. 2015;98(5):3086-99. PMid:25726114.

Savio JD, Thatcher WW, Morris GR, Entwistle K, Drost M, Mattiacci MR. Effects of induction of low plasma progesterone concentrations with a progesterone-releasing intravaginal device on follicular turnover and fertility in cattle. J Reprod Fertil. 1993;98(1):77-84. PMid:8345482.

Shabankareh HK, Habibizad J, Sarsaifi K, Cheghamirza K, Jasemi VK. The effect of the absence or presence of a corpus luteum on the ovarian follicular population and serum oestradiol concentrations during the estrous cycle in Sanjabi ewes. Small Rumin Res. 2010;93(2-3):180-5.

Sugulle AH, Dochi O, Koyama H. Developmental competence of bovine oocytes selected by brilliant cresyl blue staining: effect of the presence of corpus luteum on embryo development. J Mamm Ova Res. 2008;25(1):50-5.

Viana JHM. 2019 statistics of embryo production and transfer in domestic farm animals. Divergent trends for IVD and IVP embryos. Embryo Technology Newsletter. 2020;38(4):7-25.

Wiltbank MC, Souza AH, Carvalho PD, Bender RW, Nascimento AB. Improving fertility to timed artificial insemination by manipulation of circulating progesterone concentrations in lactating dairy cattle. Reprod Fertil Dev. 2011;24(1):238-43. PMid:22394964.

Wit AA, Wurth YA, Kruip TA. Effect of ovarian phase and follicle quality on morphology and developmental capacity of the bovine cumulus-oocyte complex. J Anim Sci. 2000;78(5):1277-83. PMid:10834583.

Yamamoto T, Iwata H, Goto H, Shiratuki S, Tanaka H, Monji Y, Kuwayama T. Effect of maternal age on the developmental competence and progression of nuclear maturation in bovine oocytes. Mol Reprod Dev. 2010;77(7):595-604. PMid:20575084.

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