Animal Reproduction (AR)
https://www.animal-reproduction.org/article/doi/10.1590/1984-3143-AR2020-0036
Animal Reproduction (AR)
Original Article

Dimethylacetamide alone or in combination with glycerol can be used for cryopreservation of ovine semen

Gabriel Felipe Oliveira de Menezes; Rodrigo Freitas Bittencourt; Fernando de Lima Cardoso; Maicon Pereira Lents; Elisiane Sateles dos Santos; Renata Oliveira Barreto; Edivânia Oliveira de Jesus; Mónica Madrigal Valverde; Antonio de Lisboa Ribeiro Filho

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Abstract

Abstract: Dimethylacetamide has been included in different extenders for the cryopreservation of semen from species with promising results. The objective of this study was to evaluate the use of dimethylacetamide (DMA) in different concentrations, associated or not with glycerol (GLY), for the cryopreservation of ovine semen, and its effects on in vitro sperm parameters and post-thaw in vivo fertility. Five semen samples of five adult Santa Ines sheep (n=25) were used. The collected ejaculates were divided among the seven treatments for subsequent cryopreservation. The treatments presented different concentrations of DMA and GLY, being divided as G1: GLY 6%; G2: DMA 3%; G3: GLY 5% + DMA 1%; G4: GLY 4% + DMA 2%; G5: GLY 3% + DMA 3%; G6: GLY 2% + DMA 4%; G7: GLY 1% + DMA 5%. %. Post-thawing of the straws, aliquots were evaluated for computerized sperm kinetics (CASA) and plasma membrane integrity, using fluorescent probes and flow cytometry. After the in vitro evaluation of the sperm parameters, in vivo testing was performed by laparoscopic artificial insemination of 72 females. The post-thaw total motility (%) evaluated by CASA were 51.4, 51.4, 50.1, 53.6, 52.3, 52.8 and 46.9, respectively, for the seven groups. And the plasma membrane integrity (%) were 19.7, 28.4, 22.3, 29.4, 24.3, 17.9 and 16.9, respectively. There were no differences (P> 0.05) between the treatments for the parameters of spermatic kinetics and membrane integrity. For females inseminated with semen from the control group (G1, GLY6%), the percentage of pregnant females was 36.1%, a result similar to that obtained with G3 treatment (GLY5% + DMA1%). In conclusion, dimethylacetamide, either alone or in combination with glycerol, can be used for cryopreservation of ovine semen.

Keywords

semen, cryoprotectants, artificial insemination, sheep

References

Alvarenga MA, Papa FO, Landim-Alvarenga FC, Medeiros ASL. Amides as cryoprotectants for freezing stallion semen: a review. Anim Reprod Sci. 2005;89(1-4):105-13. http://dx.doi.org/10.1016/j.anireprosci.2005.07.001.

Alves JP, Corcini CD, Silva EF, Caldas JS, Cardoso TF, Piedras SRN, Jardim RD, Varela AS Jr. The role of amides in seminal cryopreservation of wild silverside, Odontesthes bonariensis. Cryobiology. 2016;73(3):383-7. http://dx.doi.org/10.1016/j.cryobiol.2016.09.001.

Anel L, Kaabi M, Abroug B, Alvarez M, Anel E, Boixo JC, Fuente LF, Paz P. Factor influencing the success of vaginal and laparoscopic artificial insemination in Churra ewes: a field assay. Theriogenology. 2005;63(4):1235-47. http://dx.doi.org/10.1016/j.theriogenology.2004.07.001.

Bianchi I, Calderam K, Maschio ÉF, Madeira EM, da Rosa Ulguim R, Corcini CD, Bongalhardo DC, Corrêa ÉK, Lucia T Jr, Deschamps JC, Corrêa MN. Evaluation of amides and centrifugation temperature in boar semen cryopreservation. Theriogenology. 2008a;69(5):632-8. http://dx.doi.org/10.1016/j.theriogenology.2007.11.012.

Bianchi I, Calderam K, Maschio ÉF, Madeira EM, Ulguim RDR, Ramboi G, Corrêa ÉK, Lucia T, Deschamps JC, Corrêa MN. Intrauterine artificial insemination of gilts using cryopreserved boar semen with dimetilacetamide and glycerol. Cienc Rural. 2008b;38:1978-83. http://dx.doi.org/10.1590/S0103-84782008000700028.

Bittencourt RF, Oba E, Biscarde CEA, Azevedo HC, Bittencourt MV, Menezes GFO, Lima AS, Fuchs KM, Ribeiro Filho AL. Dimethylacetamide and trehalose for ram semen cryopreservation. Cryobiology. 2018;85:1-6. http://dx.doi.org/10.1016/j.cryobiol.2018.10.266.

Bittencourt RF, Oba E, Ribeiro Filho AL, Chalhoub M, Vasconcelos MF, Biscarde CA, Bicudo SD. Trehalose and a calcium chelator for ram semen cryopreservation. Arch Vet Sci. 2014;19(2):69-77. http://dx.doi.org/10.5380/avs.v19i2.31064.

Bittencourt RF, Ribeiro Filho AL, Alves SGG, Vasconcelos MF, Biscarde CE, Leal LS, Oba E. O efeito de um quelante de cálcio, de um detergente e da lecitina de soja sobre a congelabilidade do sêmen caprino. Braz. J. Vet. Res. An. Sci., 2008;45:305-312.

Cavalcante TV, Esper CR, Azevedo HC, Cordeiro MF. Análise Computadorizada (Casa) e convencional da Motilidade Espermática de Sêmen Caprino das Raças Boer e Alpina no Outono e Primavera. Ars Vet. 2005;21:203-8. http://dx.doi.org/10.15361/2175-0106.2005v21n4p203-208.

Cedenho AP, Tokunaga IM. Comparison of supravital staining techniques for spermatozoa: eosin and eosin-nigrosin. Jornal Brasileiro de Urologia. 1995;16:234-6.

Davis RO, Katz D. Standardization and comparability of CASA instruments. J Androl. 1992;13:81-6. http://dx.doi.org/10.1002/j.1939-4640.1992.tb01632.x.

Godfrey RW, Collins JR, Hensley EL, Wheaton JE. Estrus synchronization and artificial insemination of hair sheep ewes in the tropics. Theriogenology. 1999;51(5):985-97. http://dx.doi.org/10.1016/S0093-691X(99)00044-8.

Harrison RAP, Vickers SE. Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. J Reprod Fertil. 1990;88(1):343-52. http://dx.doi.org/10.1530/jrf.0.0880343.

Henry M, Neves JP, Jobim MIM. Manual para exame andrológico e avaliação de sêmen animal. 3. ed. Belo Horizonte: Colégio Brasileiro de Reprodução Animal; 2013. 104 p.

Hill JR, Thompson JA, Perkins NR. Factors affecting pregnancy rates following laparoscopic insemination of 28447 Merino ewes under commercial conditions. Theriogenology. 1998;49(4):697-708. http://dx.doi.org/10.1016/S0093-691X(98)00019-3.

Killen ID, Caffery GJ. Uterine insemination of ewes with the aid of a laparoscope. Aust Vet J. 1982;59(3):95. http://dx.doi.org/10.1111/j.1751-0813.1982.tb02737.x.

Ladha S. Lipid heterogeneity and membrane fluidity in a highly polarized cell, the mammalian spermatozoon. J Membr Biol. 1998;165(1):1-10. http://dx.doi.org/10.1007/s002329900415.

Li YH, Cai KJ, Kovacs A, Ji WZ. Effects of various extenders and permeating cryoprotectants on cryopreservation of Cynomolgus Monkey (Macaca fascicularis) spermatozoa. J Androl. 2005;26(3):387-95. http://dx.doi.org/10.2164/jandrol.04147.

Masoudi R, Zare Shahneh A, Towhidi A, Kohram H, Akbarisharif A, Sharafi M. Fertility response of artificial insemination methods in sheep with fresh andfrozen-thawed semen. Cryobiology. 2017;74:77-80. http://dx.doi.org/10.1016/j.cryobiol.2016.11.012.

Medeiros ASL, Gomes GM, Carmo MT, Papa FO, Alvarenga MA. Cryopreservation of stallion sperm using different amides. Theriogenology. 2002;58:1-4. http://dx.doi.org/10.1016/S0093-691X(02)00898-1.

Melo C, Zahn F, Martin I, Orlandi C, Dell’aqua J Jr, Alvarenga MA, Papa FO. Influence of semen storage and cryoprotectant on post-thaw viability and fertility of stallion spermatozoa. J Equine Vet Sci. 2007;27(4):171-5. http://dx.doi.org/10.1016/j.jevs.2007.02.008.

Mendoza N, Casao A, Del Valle I, Serrano E, Nicolau S, Asumpcao M, Muino-Blanco T, Cebrian-Perez JA, Perez-Pe R. Quality characteristics and fertilizing ability of ram sperm subpopulations separated by partition in an aqueous two-phase system. J Chromatogr B Analyt Technol Biomed Life Sci. 2012;880:74-81. http://dx.doi.org/10.1016/j.jchromb.2011.11.019.

Mosca F, Madeddu M, Sayed AA, Zaniboni L, Iaffaldano N, Cerolini S. Data on the positive synergic action of dimethylacetamide and trehalose on quality of cryopreserved chicken sperm. Data Brief. 2016;9:1118-21. http://dx.doi.org/10.1016/j.dib.2016.11.059.

Okuda Y, Seita Y, Hisamatsu S, Sonoki S, Shino M, Masaoka T, Inomata T, Kamijo S, Kashiwazaki N. Fertility of spermatozoa cryopreserved with 2% acetamide or glycerol through artificial insemination in the Japanese White Rabbits. Exp Anim. 2007;56(1):29-34. http://dx.doi.org/10.1538/expanim.56.29.

Palacín I, Vicente-Fiel S, Santolaria P, Yániz JL. Standardization of CASA sperm motility assessment in the ram. Small Rumin Res. 2013;112(1-3):128-35. http://dx.doi.org/10.1016/j.smallrumres.2012.12.014.

Pinho RO, Lima DMA, Shiomi HH, Siqueira JB, Silva HT, Lopes PS, Guimarães SEF, Guimarães JD. Effect of different cryo-protectants on the viability of frozen/thawed semen from boars of the Piau breed. Anim Reprod Sci. 2014;146(3-4):187-92. http://dx.doi.org/10.1016/j.anireprosci.2014.02.018.

Rabassa VR, Tabeleão VC, Pfeifer LFM, Schneider A, Ziguer EA, Schossler ED, Severo NC, Pino FAB, Corrêa MV. Efeito das técnicas transcervical e laparoscópica sobre a taxa de prenhez de ovelhas inseminadas em tempo fixo. Cienc Anim Bras. 2007;8:127-33.

Rosato MP, Iaffaldano N. Cryopreservation of rabbit semen: comparing the effects of different cryoprotectants, cryoprotectant-free vitrification, and the use of albumin plus osmoprotectants on sperm survival and fertility after standard vapor freezing and vitrification. Theriogenology. 2013;79(3):508-16. http://dx.doi.org/10.1016/j.theriogenology.2012.11.008.

Salamon S, Maxwell WMC. Storage of ram semen. Anim Reprod Sci. 2000;62(1-3):77-111. http://dx.doi.org/10.1016/S0378-4320(00)00155-X.

Seifi-Jamadi A, Ahmad E, Ansari M, Kohram H. Antioxidant effect of quercetin in an extender containing DMA or glycerol on freezing capacity of goat semen. Cryobiology. 2017;75:15-20. http://dx.doi.org/10.1016/j.cryobiol.2017.03.002.

Silva ECB, Cajueiro JFP, Silva SV, Vidal AH, Soares PC, Guerra MMP. In vitro evaluation of ram sperm frozen with glycerol, ethylene glycol or acetamide. Anim Reprod Sci. 2012;132(3-4):155-8. http://dx.doi.org/10.1016/j.anireprosci.2012.05.014.

Thurston LM, Holt WV, Watson PF. Post-thaw functional status of boar spermatozoa cryopreserved using three controlled rate freezers: a comparison. Theriogenology. 2003;60(1):101-13. http://dx.doi.org/10.1016/S0093-691X(02)01331-6.

Vafaei F, Kohram H, Zareh-Shahne A, Ahmad E, Seifi-Jamadi A. Influence of Different Combinations of Permeable and Nonpermeable Cryoprotectants on the Freezing Capacity of Equine Sperm. J Equine Vet Sci. 2019;75:69-73. http://dx.doi.org/10.1016/j.jevs.2019.01.014.

Varela AS Jr, Corcini CD, Gheller SMM, Jardim RD, Lucia JRT, Streit Jr DP, Figueiredo MRC. Use of amides as cryoprotectants in extenders for frozen sperm of tambaqui, Colossoma macropomum. Theriogenology. 2012;78(2):244-51. http://dx.doi.org/10.1016/j.theriogenology.2012.02.029.

Vidament M, Vincent P, Martin FX, Magistrini M, Blesbois E. Differences in ability of jennies and mares to conceive with cooled and frozen semen containing glycerol or not. Anim Reprod Sci. 2009;112(1-2):22-35. http://dx.doi.org/10.1016/j.anireprosci.2008.03.016.

Wu TW, Cheng FP, Chen IH, Yang CH, Tsai MY, Chang MH, Wang JH, Wu JT. The combinatorial effect of different Equex STM paste concentrations, cyroprotectants and the straw-freezing methods on the post-thaw boar semen quality. Reprod Domest Anim. 2013;48(1):53-8. http://dx.doi.org/10.1111/j.1439-0531.2012.02022.x.
 


Submitted date:
04/27/2020

Accepted date:
11/24/2020

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