Animal Reproduction (AR)
https://www.animal-reproduction.org/article/doi/10.1590/1984-3143-AR2022-0106
Animal Reproduction (AR)
ORIGINAL ARTICLE

Establishment of pelvic inflammatory disease model induced by vaginal injection of Ureaplasma urealyticum liquids combined with fatigue and hunger

Pengfei Liu; Xiao Yu; Jinjin Wang; Li Wang; Yi Ding; Jinxing Liu

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Abstract

Abstract: Pelvic inflammatory disease (PID) is an inflammation of the upper genital tract. PID is the leading cause of some severe sequelae in the absence of timely and accurate diagnosis and treatment. An appropriate animal model is needed to explore the underlying mechanism of PID sequelae. This study introduced an animal model of PID by vaginal injection of liquid Ureaplasma urealyticum combined with fatigue and hunger (UVF). This study was designed to test the feasibility of a rat model. A rat model was established using UVF irradiation. Levels of some inflammatory cytokines in the serum and the homogenates of the fallopian tubes were measured by ELISA, RT-PCR, and flow cytometry and compared with another rat model of Ureaplasma urealyticum liquids injected into the two uterus horns during laparotomy. Inflammatory alterations and adhesions were observed after hematoxylin and eosin (H&E) staining and detected using the Blauer scoring system. The results showed that the combined UVF and rat model caused apparent obstruction, edema, and adhesion in the fallopian tubes and connective tissues. The rat model showed upregulated CD4, CD8, and CD4/CD8 in peripheral blood mononuclear cells (PBMCs) and significantly increased levels of IL-4, IL-6, IL-10, and IL-17. UVF also enhanced the expression of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, vascular endothelial growth factor (VEGF) β, and matrix metalloproteinase (MMP)-2 (P<0.05). The UVF rat model can induce inflammatory alterations in the fallopian tubes and connective tissues, and can be used as a model of PID.

Keywords

pelvic inflammatory disease, microorganism, inflammatory cytokines, adhesion

References

Alizadeh A, Dyck SM, Kataria H, Shahriary GM, Nguyen DH, Santhosh KT, Karimi-Abdolrezaee S. Neuregulin-1 positively modulates glial response and improves neurological recovery following traumatic spinal cord injury. Glia. 2017;65(7):1152-75. http://dx.doi.org/10.1002/glia.23150. PMid:28456012.

Brown EM, Kenny DJ, Xavier RJ. Gut microbiota regulation of T cells during inflammation and autoimmunity. Annu Rev Immunol. 2019;37(1):599-624. http://dx.doi.org/10.1146/annurev-immunol-042718-041841. PMid:31026411.

Brunham RC, Gottlieb SL, Paavonen J. Pelvic inflammatory disease. N Engl J Med. 2015;372(21):2039-48. http://dx.doi.org/10.1056/NEJMra1411426. PMid:25992748.

Curry A, Williams T, Penny ML. Pelvic inflammatory disease: diagnosis, management, and prevention. Am Fam Physician. 2019;100(6):357-64. PMid:31524362.

De Clercq E, Kalmar I, Vanrompay D. Animal models for studying female genital tract infection with Chlamydia trachomatis. Infect Immun. 2013;81(9):3060-7. http://dx.doi.org/10.1128/IAI.00357-13. PMid:23836817.

Feng S, Gao L, Zhang D, Tian X, Kong L, Shi H, Wu L, Huang Z, Du B, Liang C, Zhang Y, Yao R. MiR-93 regulates vascular smooth muscle cell proliferation, and neointimal formation through targeting Mfn2. Int J Biol Sci. 2019;15(12):2615-26. http://dx.doi.org/10.7150/ijbs.36995. PMid:31754334.

Haggerty CL, Totten PA, Tang G, Astete SG, Ferris MJ, Norori J, Bass DC, Martin DH, Taylor BD, Ness RB. Identification of novel microbes associated with pelvic inflammatory disease and infertility. Sex Transm Infect. 2016;92(6):441-6. http://dx.doi.org/10.1136/sextrans-2015-052285. PMid:26825087.

Holtmann MH, Neurath M. Differential TNF-signaling in chronic inflammatory disorders. Curr Mol Med. 2004;4(4):439-44. http://dx.doi.org/10.2174/1566524043360636. PMid:15354874.

Ilatovskaya DV, Pitts C, Clayton J, Domondon M, Troncoso M, Pippin S, DeLeon-Pennell KY. CD8+ T-cells negatively regulate inflammation post-myocardial infarction. Am J Physiol Heart Circ Physiol. 2019;317(3):H581-96. http://dx.doi.org/10.1152/ajpheart.00112.2019. PMid:31322426.

Iturra PA, Rojas DA, Pérez FJ, Méndez A, Ponce CA, Bonilla P, Bustamante R, Rodríguez H, Beltrán CJ, Vargas SL. Progression of type 2 helper T cell-type inflammation and airway remodeling in a rodent model of naturally acquired subclinical primary pneumocystis infection. Am J Pathol. 2018;188(2):417-31. http://dx.doi.org/10.1016/j.ajpath.2017.10.019. PMid:29169991.

Jaafari A, Baradaran Rahimi V, Vahdati-Mashhadian N, Yahyazadeh R, Ebrahimzadeh-Bideskan A, Hasanpour M, Iranshahi M, Ehtiati S, Rajabi H, Mahdinezhad M, Rakhshandeh H, Askari VR. Evaluation of the therapeutic effects of the hydroethanolic extract of portulaca oleracea on surgical-induced peritoneal adhesion. Mediators Inflamm. 2021;2021:8437753. http://dx.doi.org/10.1155/2021/8437753. PMid:34381307.

Miossec P, Kolls JK. Targeting IL-17 and TH17 cells in chronic inflammation. Nat Rev Drug Discov. 2012;11(10):763-76. http://dx.doi.org/10.1038/nrd3794. PMid:23023676.

Mitchell RE, Hassan M, Burton BR, Britton G, Hill EV, Verhagen J, Wraith DC. IL-4 enhances IL-10 production in Th1 cells: implications for Th1 and Th2 regulation. Sci Rep. 2017;7(1):11315. http://dx.doi.org/10.1038/s41598-017-11803-y. PMid:28900244.

Molema G, Zijlstra JG, van Meurs M, Kamps JAAM. Renal microvascular endothelial cell responses in sepsis-induced acute kidney injury. Nat Rev Nephrol. 2022;18(2):95-112. http://dx.doi.org/10.1038/s41581-021-00489-1. PMid:34667283.

Monteiro PA, do Prado WL, dos Santos Tenório TR, Tomaz LM, St-Pierre DH, Lira FS. Immunometabolic changes in hepatocytes arising from obesity and the practice of physical exercise. Curr Pharm Des. 2018;24(27):3200-9. http://dx.doi.org/10.2174/1381612824666180515115550. PMid:29766791.

Oh Y, Lee J, Kim HC, Hahn TW, Yoon B, Han JH, Kwon YS, Park JJ, Koo DB, Rhee KJ, Jung BD. Establishment of hydrochloric acid/lipopolysaccharide-induced pelvic inflammatory disease model. J Vet Sci. 2016;17(3):413-9. http://dx.doi.org/10.4142/jvs.2016.17.3.413. PMid:26726020.

Ravel J, Moreno I, Simón C. Bacterial vaginosis and its association with infertility, endometritis, and pelvic inflammatory disease. Am J Obstet Gynecol. 2021;224(3):251-7. http://dx.doi.org/10.1016/j.ajog.2020.10.019. PMid:33091407.

Ross J, Guaschino S, Cusini M, Jensen J. 2017 European guideline for the management of pelvic inflammatory disease. Int J STD AIDS. 2018;29(2):108-14. http://dx.doi.org/10.1177/0956462417744099. PMid:29198181.

Saad S, Bendall LJ, Gottlieb DJ, Bradstock KF, Overall CM. Cancer cell-associated fibronectin induces release of matrix metalloproteinase-2 from normal fibroblasts. Cancer Res. 2002;62(1):283-9. PMid:11782389.

Sheldon IM, Rycroft AN, Dogan B, Craven M, Bromfield JJ, Chandler A, Roberts MH, Price SB, Gilbert RO, Simpson KW. Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and cause pelvic inflammatory disease in cattle and mice. PLoS One. 2010;5(2):e9192. http://dx.doi.org/10.1371/journal.pone.0009192. PMid:20169203.

Tan JTH, Suyapto DR, Neo EL, Leong PSK. Prospective audit of laparoscopic cholecystectomy experience at a secondary referral centre in South Australia. ANZ J Surg. 2006;76(5):335-8. http://dx.doi.org/10.1111/j.1445-2197.2006.03721.x. PMid:16768693.

Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014;6(10):a016295. http://dx.doi.org/10.1101/cshperspect.a016295. PMid:25190079.

Tang L, Xu Y, Wei Y, He X. Uric acid induces the expression of TNF-α via the ROS-MAPK-NF-κB signaling pathway in rat vascular smooth muscle cells. Mol Med Rep. 2017;16(5):6928-33. http://dx.doi.org/10.3892/mmr.2017.7405. PMid:28901421.

Taylor RN, Yu J, Torres PB, Schickedanz AC, Park JK, Mueller MD, Sidell N. Mechanistic and therapeutic implications of angiogenesis in endometriosis. Reprod Sci. 2009;16(2):140-6. http://dx.doi.org/10.1177/1933719108324893. PMid:19001553.

Tham E, Gielen AW, Khademi M, Martin C, Piehl F. Decreased expression of VEGF-A in rat experimental autoimmune encephalomyelitis and in cerebrospinal fluid mononuclear cells from patients with multiple sclerosis. Scand J Immunol. 2006;64(6):609-22. http://dx.doi.org/10.1111/j.1365-3083.2006.01851.x. PMid:17083617.

Vanrompay D, Lyons JM, Morré SA. Animal models for the study of Chlamydia trachomatis infections in the female genital infection. Drugs Today (Barc). 2006;42(Suppl A):55-63. PMid:16683045.

Wilson RB. Hypoxia, cytokines and stromal recruitment: parallels between pathophysiology of encapsulating peritoneal sclerosis, endometriosis and peritoneal metastasis. Pleura Peritoneum. 2018;3(1):20180103. http://dx.doi.org/10.1515/pp-2018-0103. PMid:30911653.

Zhang X, Wang J, Xing Y, Gong L, Li H, Wu Z, Li Y, Wang J, Wang Y, Dong L, Li S. Effects of ginsenoside Rg1 or 17β-estradiol on a cognitively impaired, ovariectomized rat model of Alzheimer’s disease. Neuroscience. 2012;220:191-200. http://dx.doi.org/10.1016/j.neuroscience.2012.06.027. PMid:22728092.
 


Submitted date:
11/05/2022

Accepted date:
09/12/2023

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